Carboxypeptidase B (3.4.17.2) is a metallo-carboxypeptidase that catalyses the hydrolysis of the basic amino acids, lysine and arginine from the C-terminal position of polypeptides. Recombinant Carboxypeptidase B is expressed in E. coli and provides an alternative to animal origin Carboxypeptidase B. The synthetic gene cloning rules out any possibility of pathogenic agents and related non-specific enzymes. The major application for Carboxypeptidase B is manufacturing of recombinant proteins such as insulin and insulin analogs. Other applications include peptide mapping, protein sequencing, acute pancreatitis diagnosis, cytochrome C stepwise digestion, etc. Its high purity, high specificity and Animal Origin Free (AOF) quality makes it the most desirable form of Carboxypeptidase B available.

At BioGenomics GMP Grade Recombinant Carboxypeptidase B is available as CarboxyB – AOF (Proteomics).

Recombinant Enterokinase Light chain (3.4.21.9) is the catalytic subunit of bovine enterokinase, which is expressed in E. coli and purified to yield a high enzyme activity preparation. The synthetic bovine gene cloning rules out any possibility of pathogenic agents and related non-specific enzymes. EK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. However, Enterokinase does not act when Lysine is followed by Proline. The enzyme can be used to cleave any fusion protein that carries this sequence. Its high purity, high specificity and Animal Origin Free (AOF) quality makes it the most desirable form of Enterokinase available.

At BioGenomics GMP Grade Recombinant Enterokinase is available as EKDK – AOF (Proteomics).

Enzymes such as Trypsin that are used in Biopharmaceutical manufacturing for modification or activation of products must be removed before the final release of the product as they may pose a safety risk. Conventional enzyme activity assays fail to detect inactivated nonetheless immunogenic enzyme. BioGenomics’ ELISA based Residual Trypsin detection kit includes high affinity polyclonal antibodies that detect the presence of these enzymes (intact and/or fragmented) with a high sensitivity and specificity. Independent of its activeness whether intact or fragmented these enzymes are reliably detected to ensure product safety.

Antibodies are raised specifically against BioGenomics’ Recombinant Trypsin. Combining these kits with BioGenomics limited AOF GMP grade Trypsin, augments the production efficiency from the use of high-activity enzymes to its timely verification of clearance.

Enzymes such as Carboxypeptidase B (CPB) that are used in Biopharmaceutical manufacturing for modification or activation of products must be removed before the final release of the product as they may pose a safety risk. Conventional enzyme activity assays fail to detect inactivated nonetheless immunogenic enzyme. BioGenomics’ ELISA based Residual CPB detection kit includes high affinity polyclonal antibodies that detect the presence of these enzymes (intact and/or fragmented) with a high sensitivity and specificity. Independent of its activeness whether intact or fragmented these enzymes are reliably detected to ensure product safety.

Antibodies are raised specifically against BioGenomics recombinant CPB. Combining these kits with BioGenomics
limited AOF GMP grade CPB, augments the production efficiency from the use of high-activity enzymes to its timely verification of clearance.

The host cells used for recombinant expression contain hundreds to thousands of host cell proteins (HCPs) that can contaminate biopharmaceutical products. Failure to sufficiently reduce impurities early in drug development can reduce drug efficacy or lead to adverse patient reactions. Regulations require reduction of HCPs to the lowest levels possible. The ELISA Host Cell Protein (HCP) detection kit allows you to identify the presence of HCP impurities in your therapeutic at any phase of your product’s development. These kits can be used during the protein purification process to monitor and optimize that step of the bioproduction workflow. BioGenomics’ HCP detection kits are based on a sandwich ELISA test in a 96-well plate format for E. coli expression system. Each kit allows quantification of up to 24 samples and offers a high range of sensitivity detection, antibody coverage, and minimal lot-to-lot variation.